Monday, August 29, 2022

Chapter 3: Repetitive DNA and Mobile Genetic Elements

Introduction
Half of our genome is composed of highly repetitive DNA and moderately repetitive DNA. Satellite DNA. C0t curves. (pp. 57-58)
[Transcription activity in repeat regions of the human genome]
Centromeres
Centromeres contain highly repetitive DNA. (p. 58)
[The structures of centromeres]
Telomeres

Telomeres at the ends of chromosomes contain repetitive DNA. (pp. 58-59)

   Box: Dead centromeres and telomeres (pp. 59-60)

Short tandem repeats (STRs)

Short tandem repeats (STRs) are short stretches of repetitive DNA. (p. 60)

   Box: DNA fingerprints (pp. 60-61)

Mobile genetic elements
Moderately repetitive DNA consists of interspersed copies of viruses and transposons. (p. 61)
Hidden viruses in your genome
The human genome contains copies of DNA viruses and RNA viruses. Most of them are due to ancient insertions and the viral genomes have acquired inactivating mutations. Many virus-related sequences are just fragments of the original virus genome. (pp. 61-65)
What do we need to know about transposons?
The two main tpes of transposons are DNA transposons and RNA transposons (retrotransposons). (pp. 65-67)
LINES and SINES
Long interspersed elements (LINEs) are transposons that carry a gene for reverse transcriptase. Most LINE-related sequences are degenerate versions of a once-active transposons. Short interspersed elements are derived from small noncoding genes and they require exogenous reverse transcriptase to propagate. Alu elements are one example of a SINE and there are more than one million copies in the human genome. (pp. 67-70)
How much of our genome is composed of transposon-related sequences?
Most of the transposon-related sequences are inactive fragments of the original transposons. It's diffficult to get a precise estimate of the total amount of transposon-related sequences but it's probably at least 50% of the human genome.(pp. 70-72)

   BOX: What does the humped bladderwort tell us about junk DNA? (p. 72)

Selfish genes and selfish DNA
Selfish DNA refers to DNA sequences that can propagate by themselves within the genome. (p. 73)
[Junk DNA and selfish DNA] [The selfish gene vs the lucky allele]
Exaptation versus the post hoc fallacy
Some transposon-related sequences have secondarily acquired a function that contributes to the fitness of the organism. This is an example of exaptation. Some scientists believe that transposon-related sequences are retained in order to serve as a reservoir for future exaptation but this argment is related to a logical fallacy called the post hoc fallacy. (pp. 73-78)
[Peter Larsen: "There is no such thing as 'junk DNA'"]
Mitochondria are invading your genome!
The human genome contains fragments of mitochondrial DNA that have recently been incorprated by accident. (pp. 78-79)
[How much mitochondrial DNA in your genome?]
On the origin of junk DNA

A lot of junk DNA originates from ancient insertions of transposons and their subsequent degeneration by acquiring mutations. (pp. 79-80)

If it walks like a duck ...

Transposons look like junk, behave like junk, and evolve like junk, so let's just call them junk. (pp. 80-81)

Notes for Chapter 3 (pp. 320-321)

Thursday, August 11, 2022

Chapter 1: Introducing Genomes

Introduction
The discovery of DNA structure and the structure of nuleotides. Defining the 5′ and 3′ ends. (pp. 7-9)
The double helix
The structure of polynucleotides and the double helix. Base pairs, stacking interactions, and hydrogen bonds. (pp. 9-13)
The goal of the human genome project was to sequence all of the base pairs
Writing DNA sequences. (pp. 13-14)
Prokaryotes and eukaryotes
Differences between prokaryotes and eukaryotes. Bacteria vs prokaryotes. The Age of Bacteria. "Higher" vs "lower." (pp. 14-16)
[We live in the age of bacteria]
How big is your genome?

Historical estimates of the weight of DNA (3.5 pg). Calculating the number of base pairs (3.2 × 109 bp). Length of the genome. (pp. 16-18)
[How big is the human genome (2023)?] [Genome size confusion]

Packaging DNA: nucleosomes and chromatin
Histones, core particles, nucleosomes, and chromatin. Heterochromatin and euchromatin. Sequencing the euchromatic genome. (pp. 19-21)
Transcription
"A gene is a DNA sequence that's transcribed to produce a functional product." Transcription initiation, elongaton, termination. (pp. 21-24)
Translation
Messenger RNA. Gene orientation: template strand, coding strand. Initiation, elongation (peptide bond), termination. (pp. 24-27)
The genetic code
Aminacylated tRNA. Standard genetic code. (pp. 27-29)
Introns and exons
Protein-coding genes, noncoding genes. RNA processing, splicing, spliceosome. (pp. 29-32)
Notes for Chapter 1 (pp. 317-318)

Chapter 2: The Evolution of Sloppy Genomes

Introduction
Pufferfish, lungfish, frogs, and the C-Value Paradox. (pp. 33-34)
The complexity of genomes
Reassociation kinetics (C0t curves). Highly repetitive DNA, moderately repetitive DNA, unique sequence DNA. (pp. 34-35)
Variation in genome size
Junk DNA explains the variations in genome size. The C-Value Enigma. You don't need new genes to explain complexity. (pp. 35-37)
Instantaneous genome doubling
Polyploidy. Brassica species. Organisms can tolerate extra DNA. (pp. 38-39)
The Onion Test

The Onion test is a way of testing your junk DNA hypotheses. (pp. 39-40)
[The Onion Test]

Modern evolutionary theory

Evolution is a change in the frequency of alleles in a population. Adaptation, fixation, postive selection, negative selection, purifying selection. (pp. 40-41)
[Is the Modern Synthesis effectively dead?] [Kevin Laland's view of "modern" evolutionary theory (again)]

Random genetic drift
Beanbag genetics and population genetics. Fixation by random genetic drift. (pp. 41-43)
[On the importance of random genetic drift in modern evolutionary theory] [Evolution by chance] [The role of chance in evolution] [One philosopher's view of random genetic drift] [A philosopher's view of random genetic drift]
Neutral Theory

Kimura and the promotion of the neutral theory. Random genetic drift as a major cause of evolution. (pp. 43-44)
[Celebrating 50 years of Neutral Theory]

Nearly neutral Theory
Ohta and the nearly neutral Theory. The fixation of slightly deleterious alleles.(pp. 44-45)
Population genetics

Population size and selection coefficients. Probability of fixation. Population size and the fixation of slightly deleterious alleles. Drift-Barrier Hypothesis. (pp. 45-49)
[Learning about modern evolutionary theory: the drift-barrier hypothesis]

   Box: Are humans are still evolving? (pp. 49-50)

On the evolution of sloppy genomes

Insertions, deletions, and random genetic drift. (pp. 50-54)
[Evolution by Accident]

   Box: Chromosome dynamics (p. 54)
   [Segmental duplications in the human genome]

Bacteria have small genomes
Why do bacteria have small genomes? (pp. 54-56)

Notes for Chapter 2 (pp. 318-320)